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Bruce A. McClane, PhD

Dr. Bruce McClane


Fax: 412-624-2139
420 Bridgeside Point II
450 Technology Drive


Postdoc, NYU School of Medicine

PhD in Microbiology and Cell Biology, Penn State University

Academic Affiliation(s)

Professor, Department of Microbiology and Molecular Genetics


Our laboratory studies bacterial pathogenesis. Specifically, we focus on enteric diseases caused by Clostridium perfringens type A (including C. perfringens type A food poisoning, which is the second most common bacterial foodborne illness in the USA and several nonfoodborne human GI illnesses, notably antibiotic-associated diarrhea and sporadic diarrhea), and several veterinary enterotoxemias caused by type B-D isolates. Type B and D infections are of particular interest because they involve epsilon toxin, a class B CDC/USDA overlap select toxin.

Lab Personnel

Jihong Li, Research Instructor

Jianming Chen, Research Associate

Menglin Ma, Research Associate

Archana Shrestha, Research Associate

James Theoret, Postdoc

John Courtland Freedman, Postdoc

Areas of Interest

Bacterial toxins, bacterial pathogenesis, regulation of virulence, toxin plasmids


Vidal J. E, Ma M, Saputo J, Garcia J, Uzal F. A, and McClane B. A. Evidence that the Agr-like quorum sensing system regulates the toxin production, cytotoxicity and pathogenicity of Clostridium perfringens type C isolate CN3685. Mol Microbiol. 83: 179-194. |  View Abstract

Ma M, Vidal J, Saputo J, McClane B. A, and Uzal F. The VirS/VirR two-component system regulates the anaerobic cytotoxicity, intestinal pathogenicity, and enterotoxemic lethality of Clostridium perfringens type C isolate CN3685. MBio. 2: e00338-10. |  View Abstract

Li J, Sayeed S, Robertson S, Chen J, and McClane B. A. Sialidases affect the host cell adherence and epsilon toxin-induced cytotoxicity of Clostridium perfringens type D strain CN3718. PLoS Pathog. 7: e1002429. |  View Abstract

Chen J, Rood J. I, and McClane B. A. Epsilon-toxin production by Clostridium perfringens type D strain CN3718 is dependent upon the agr operon but not the VirS/VirR two-component regulatory system. MBio. 2: e00275-11. |  View Abstract

Briggs D. C, Naylor C. E, Smedley J. G, 3rd, Lukoyanova N, Robertson S, Moss D. S, McClane B. A, and Basak A. K. Structure of the food-poisoning Clostridium perfringens enterotoxin reveals similarity to the aerolysin-like pore-forming toxins. J Mol Biol. 413: 138-149. |  View Abstract

Vidal J. E, Ohtani K, Shimizu T, and McClane B. A. Contact with enterocyte-like Caco-2 cells induces rapid upregulation of toxin production by Clostridium perfringens type C isolates. Cell Microbiol. 11: 1306-1328. |  View Abstract

Li J, and McClane B. A. A novel small acid soluble protein variant is important for spore resistance of most Clostridium perfringens food poisoning isolates. PLoS Pathog. 4: e1000056 |  View Abstract