Graham Hatfull, PhD

Contact

gfh@pitt.edu

412-624-6975

Fax: 412-624-4870

376 Crawford Hall

Fifth and Ruskin Avenues

Education

Postdoctoral, Yale University and MRC (LMB) Cambridge (UK)

PhD in Molecular Biology, University of Edinburgh (Scotland)

BSc (Hons.) in Biological Sciences, University of London (UK)

Academic Affiliation(s)

Professor, Department of Biological Sciences

Eberly Family Professor of Biotechnology

HHMI Professor

Member, Molecular Virology and Microbiology Graduate Program

Research

Dr. Hatfull’s research focuses on the molecular genetics of the mycobacteria and their bacteriophages. These studies take advantage of the intimacy of phage-host interactions to gain insights into the genetics and physiology of Mycobacterium tuberculosis, the causative agent of human TB. Through integrated research-education programs such as the PHIRE and SEA-PHAGES programs, the large collection of completely sequenced mycobacteriophage genomes provides insights into viral diversity and evolution, and represents a rich toolbox of new approaches to understanding M. tuberculosis. Development of vector systems, selectable markers, recombineering approaches, expression tools, and insights into mycobacterial biofilms reflect some of the useful applications of this genomic resource.

Lab Personnel

Pallavi Ghosh – Research Assistant Professor
Welkin Pope - Research Assistant Professor
Gregory Broussard – Postdoc
Rebekah Dedrick – Postdoc
Shweta Singh – Postdoc
Deborah Jacobs-Sera – HHMI Coordinator
Daniel Russell – Lab Instructor
Lauren Oldfield – Grad Student
Valerie Villanueva – Grad Student
Ching-Chung Ko – Grad Student
Bryce Lunt – Grad Student
Carlos Guerrero – Staff
Zaritza Petrova – Staff
Charles Bowman – Staff
Crystal Petrone – Staff
Michelle Boyle – GSPH Student
Kathleen Pulice – Undergrad
Kate Rochenbach – Undergrad
Zane Foster – Undergrad
Enoch Tse – Undergrad
Abigail McPherson – Undergrad
Emilee Shine – Undergrad
Victoria Schneider – Undergrad
Phil Williams – Undergrad
Alexandra Cathcart – Undergrad
Terence Parker – Undergrad
Michael Ward – Undergrad
Forrest Guilfoile – Undergrad
Matthew Olm - Undergrad

Areas of Interest

Mycobacteria; bacteriophages; tuberculosis; genomics

Publications

Payne K. M, and Hatfull G. F. Mycobacteriophage endolysins: diverse and modular enzymes with multiple catalytic activities. PLoS One. 7: e34052. | View Abstract

Pope W. H, Ferreira C. M, Jacobs-Sera D, Benjamin R. C, Davis A. J, DeJong R. J, Elgin S. C, Guilfoile F. R, Forsyth M. H, Harris A. D, Harvey S. E, Hughes L. E, Hynes P. M, Jackson A. S, Jalal M. D, MacMurray E. A, Manley C. M, McDonough M. J, Mosier J. L, Osterbann L. J, Rabinowitz H. S, Rhyan C. N, Russell D. A, Saha M. S, Shaffer C. D, Simon S. E, Sims E. F, Tovar I. G, Weisser E. G, Wertz J. T, Weston-Hafer K. A, Williamson K. E, Zhang B, Cresawn S. G, Jain P, Piuri M, Jacobs W. R, Jr., Hendrix R. W, and Hatfull G. F. Cluster K mycobacteriophages: insights into the evolutionary origins of mycobacteriophage TM4. PLoS One. 6: e26750. | View Abstract

Cresawn S. G, Bogel M, Day N, Jacobs-Sera D, Hendrix R. W, and Hatfull G. F. Phamerator: a bioinformatic tool for comparative bacteriophage genomics. BMC Bioinformatics. 12: 395. | View Abstract

Marinelli L. J, Piuri M, Swigonova Z, Balachandran A, Oldfield L. M, van Kessel J. C, and Hatfull G. F. BRED: a simple and powerful tool for constructing mutant and recombinant bacteriophage genomes. PLoS One. 3: e3957. | View Abstract

van Kessel J. C, and Hatfull G. F. Recombineering in Mycobacterium tuberculosis. Nat Methods. 4: 147-152. | View Abstract

Ojha A, Anand M, Bhatt A, Kremer L, Jacobs W. R, Jr., and Hatfull G. F. GroEL1: a dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria. Cell. 123: 861-873. | View Abstract

Pedulla M. L, Ford M. E, Houtz J. M, Karthikeyan T, Wadsworth C, Lewis J. A, Jacobs-Sera D, Falbo J, Gross J, Pannunzio N. R, Brucker W, Kumar V, Kandasamy J, Keenan L, Bardarov S, Kriakov J, Lawrence J. G, Jacobs W. R, Jr., Hendrix R. W, and Hatfull G. F. Origins of highly mosaic mycobacteriophage genomes. Cell. 113: 171-182. | View Abstract